Signal enhancement in ESR spin-trapping for hydroxyl radicals.
نویسندگان
چکیده
In an ESR spin-trapping measurement of hydroxyl radicals formed in a Fenton reaction, the trapping efficiency with DMPO increased by about 300 times in a sodium trifluoroacetate solution, whereas it was little changed in a phosphate buffer.
منابع مشابه
ESR spin trapping studies of free radicals generated from nitrofuran derivative analogues of nifurtimox by electrochemical and Trypanosoma cruzi reduction.
Electron spin resonance (ESR) spectra of radicals obtained from two analogues of the antiprotozoal drug nifurtimox by electrolytic and Trypanosoma cruzi reduction were analyzed. The electrochemistry of these compounds was studied using cyclic voltammetry. STO 3-21G ab initio and INDO molecular orbital calculations were performed to obtain the optimized geometries and spin distribution, respecti...
متن کاملVanadyl-induced Fenton-like reaction in RNA. An ESR and spin trapping study.
Vanadyl (VO2+) complexed to RNA reacts with hydrogen peroxide in a Fenton-like manner producing hydroxyl radicals (.OH). The hydroxyl radicals can be spin trapped with 5,5-dimethyl-1-pyrroline-1-oxide (DMPO) forming the DMPO-OH spin adduct. In addition, in the presence of ethanol the formation of the hydroxyethyl radical adduct of DMPO (DMPO-ETOH) confirms the production of hydroxyl radicals by...
متن کاملProduction of oxygen-centered radicals by neutrophils and macrophages as studied by electron spin resonance (ESR).
Neutrophils and macrophages undergo a respiratory burst and an increase in the activity of the hexose monophosphate pathway in response to particulate or soluble agents. The increase in oxygen consumption was found to be associated with the production of oxygen-centered radicals. The ESR technique of spin trapping showed that besides a superoxide spin adduct, a hydroxyl spin adduct is also prod...
متن کاملEnhancement of Hydroxyl Radical Formation from Superoxide Anion Radical in the Presence of Organic Selenium Compounds
The generation of superoxide anion or hydroxyl radical derived from the organic selenium compounds selenomethionine, selenoethionine, selenocystine, selenocystamine and selenocysteine-glutathione selenenyl sulfide (CySeSG) was investigated by the electron spin resonance (ESR) technique with 5,5-dimethyl-1-pyrroline Noxide (DMPO) as a spin trapping agent. The intensity of ESR signals of DMPO-OOH...
متن کاملSerum Hydroxyl Radical Scavenging Capacity as Quantified with Iron-Free Hydroxyl Radical Source
We have developed a simple ESR spin trapping based method for hydroxyl (OH) radical scavenging-capacity determination, using iron-free OH radical source. Instead of the widely used Fenton reaction, a short (typically 5 seconds) in situ UV-photolysis of a dilute hydrogen peroxide aqueous solution was employed to generate reproducible amounts of OH radicals. ESR spin trapping was applied to quant...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
دوره 19 7 شماره
صفحات -
تاریخ انتشار 2003